COLLABORATIVE CENTER FOR MULTIPLEXED PROTEOMICS

While the proteomics field has experienced substantial growth in the last decade, most studies have been limited in scope due in part to complex technical hurdles (i.e. lack of proteome coverage, sample sizes typically below 20, limited reference database selection, peptide assignment to orthologous proteins). The introduction of new methods and the use of the Orbitrap mass analyzers have dramatically increased the number of quantifiable proteins, allowing for a greater than 20-fold increased coverage of the proteome in the past few years. Our group has leveraged Tandem Mass Tag (TMT) technology, allowing higher throughput by multiplexing up to 16 samples without the necessity of culturing. This TMT workflow allows for an unprecedented depth of proteome data, dropping sparsity of the data from 80% in traditionally acquired label-free metaproteome studies to under 20% using our methods. Further, we utilize multinotch MS3 quantitation, which provides high sensitivity while removing signal interference of previous label-based approaches allowing for accurate quantitation

 

The COLLABORATIVE CENTER FOR MULTIPLEXED PROTEOMICS provides the following services:

 

 

1. TMT Quantitative protein profiling from different sample origins

 

2. TMT Quantitative Immunoprecipitation analysis

 

3. TMT Phosphoproteomics analysis

 

4. Single Mass Spectrometry Run (label-free)

 

 

We have expertise in processing human and murine samples that include organ tissues, blood, fecal material, and tissue culture models.

 

 

Please contact Dr. Gonzalez for pricing: djgonzalez@health.ucsd.edu